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Here, we tested the effects of mosquito age, sex, blood feeding status, and potential for horizontal transmission to investigate the potential for these factors to help or hinder Ag DNV spread in natural populations. gambiae Moss55 cells were cultured in Schneider’s medium (Sigma) supplemented with 10% fetal bovine serum (FBS) at 28 °C. gambiae mosquitoes (Keele strain) were reared at 28 °C and 80% relative humidity with h light:dark photoperiod and offered 10% sugar at all times.
Female adult mosquitoes were blood fed on commercially purchased expired human blood through artificial feeding system. During experiments, mosquitoes were reared on sugar except in cases where the effect of bloodfeeding was being specifically examined.
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Female mosquitoes were more permissive to viral infection than males.
Despite low viral titers, infected males were able to venereally transmit virus to females during mating, where the virus was localized with the transferred sperm in the spermathecae.
Understanding the interaction between Ag DNV and An.
gambiae is critical for using Ag DNV in a basic and applied manner for Anopheles gene manipulation.
Here, we tested the effects of mosquito age, sex, blood feeding status, and potential for horizontal transmission using an enhanced green fluorescent protein (EGFP) reporter Ag DNV system.